Peptide reconstitution calculator
Enter the milligrams on the vial and the millilitres of diluent you add. The calculator returns the stock concentration and shows the vial filling to scale.
These tools do not provide dosing guidance and are not for human or veterinary use. They are for research-use laboratory calculations only. Verify every result independently.
Watch the vial fill and the syringe draw.
Type the numbers on your vial. The pictures update to scale: the diluent you add, the concentration that produces, and the syringe volume that holds a chosen amount of material.
Lab aliquot, not a dose.
concentration = mg ÷ mL · µg per unit = (mg/mL × 1000) ÷ 100 · units = µg ÷ µg per unit · draws = floor(mg × 1000 ÷ µg)
Research-use laboratory arithmetic for a U-100 insulin syringe (1 unit = 0.01 mL). Not dosing guidance; not for human or veterinary use.
Adding 2 mL to 5 mg gives 2.5 mg/mL.
On a U-100 syringe each unit is 0.01 mL, so one unit holds 25 µg.
Reconstitution concentration
Concentration of a lyophilized vial after adding diluent.
concentration (mg/mL) = vial contents (mg) ÷ diluent volume (mL)
Vial fills as you type
How it works
Reconstitution is the step where a freeze-dried (lyophilized) peptide powder is dissolved in a diluent, usually bacteriostatic water, to make a stock solution. The only arithmetic involved is a division: the mass in the vial divided by the volume you add.
Because research work is usually measured in micrograms and microlitres, the result is also shown in µg/mL and as micrograms per unit of a U-100 insulin syringe, where one unit is 0.01 mL. These are unit conversions, not recommendations.
Step by step
- Read the mass on the vial label, in milligrams.
- Decide how much diluent to add, in millilitres.
- Divide mass by volume to get mg/mL.
- Multiply by 1,000 for µg/mL; divide that by 100 for µg per syringe unit.
Frequently asked
Divide the milligrams of peptide in the vial by the millilitres of diluent added. A 5 mg vial reconstituted with 2 mL gives 5 ÷ 2 = 2.5 mg/mL, which is 2,500 µg/mL.
A U-100 insulin syringe holds 1 mL across 100 units, so one unit is 0.01 mL. Multiply the concentration in µg/mL by 0.01. At 2,500 µg/mL, one unit contains 25 µg.
No. The total mass stays the same; only the concentration changes. More diluent means a weaker solution and larger volumes for the same amount of material.
Concentration (mg/mL) = vial contents (mg) ÷ diluent volume (mL). Every result on this page prints that formula beneath it.
No. Amiravo publishes laboratory arithmetic for research use only. It does not provide dosing, and nothing on this site is for human or veterinary use.