Bioregulators · Dosing reference

Pinealon dosage

What the human evidence does and does not show, how long studies ran, and the arithmetic for working with vials.

Not approved — research use onlyCPreclinical researchNone established

Full research page Cycle-length evidence Compare Prefer Amiravo on Google

Regulatory status: Not approved — research use only
Human amounts: None established in humans
Route studied: Oral (capsule)
Frequency: Not established
Half-life: Not established
Evidence grade: Grade C · Preclinical research
Vial sizes on the market: 20 mg
Other forms seen: Liquid spray 10 mL (10 mg) · Oral capsules (strength not stated)

What Pinealon is

Pinealon is a short synthetic tripeptide of the bioregulator class, studied in experimental models of neuronal and cellular aging.

Pinealon's investigators describe two overlapping explanations: a direct antioxidant or redox-modulating action, and an epigenetic action in which the tripeptide reaches the nucleus and influences gene expression ^1,3. Redox and signalling observations In cerebellar granule cells, neutrophils and PC12 cells exposed to oxidative stress induced by receptor-dependent and receptor-independent means, pinealon was… How it works →

Key evidence

Khavinson et al., Rejuvenation Research · 2011 · Cerebellar granule cells, neutrophils and PC12 cells (in vitro)
Pinealon was reported to restrict reactive oxygen species accumulation and to reduce necrotic cell death, with a delayed ERK 1/2 activation time course and cell cycle modification. ¹
Arutjunyan et al., International Journal of Clinical and Experimental Medicine · 2012 · Rats (prenatal hyperhomocysteinaemia from dietary methionine loading in pregnancy) and their offspring
Offspring of treated dams were reported to show better spatial orientation and learning and cerebellar neurons with less reactive oxygen species accumulation and fewer necrotic cells. ²
Khavinson et al., Molecules (review) · 2020 · Review of in vitro and animal studies
Summarises reports that the EDR peptide activates antioxidant enzyme synthesis in rat cerebellar neuron culture, interferes with dendritic spine loss in neuronal cultures from Alzheimer's and Huntington's disease models, and normalises behavioural responses in animals; proposes that the peptide binds histones or nucleic acids. ³

All studies on the research page

What the human evidence shows

No approved or established human dose

We searched PubMed and ClinicalTrials.gov and found no human studies that administered Pinealon. The research described on its page is from cells or animals, and animal amounts do not translate to people.

How long did human studies run?

The locomotive-crew study administered Pinealon for two weeks ^11.

Cycle-length evidence for Pinealon

Who was excluded from the studies

The indexed studies enrolled adults (workers, or people aged 41-83 years); no study in children, pregnancy or lactation was identified ^11,12.

Pinealon draw calculator

Pick the vial strength and the water added, enter an amount, and see the concentration, the units to draw and how many draws the vial holds. Arithmetic only.

1 · Vial strength

sizes on the market

2 · Water added

3 · Amount per draw

4 · Syringe

Your mix

20 mg + 2 mL water = 10 mg/mL

10 units holds 1 mg

Draw toEnter an amount in step 3

U-100 syringe · 100 units = 1 mL

Computed from your inputs. Not a dose or a recommendation.

Compare water volumes for this vialShow

More water = lower concentration = a larger, easier-to-measure draw. Tap a row to use it.

Water	mg/mL	Draw
0.5 mL	40	—
1 mL	20	—
1.5 mL	13.33	—
2 mL	10	—
2.5 mL	8	—
3 mL	6.667	—
5 mL	4	—

Supplies and handling

Lyophilized vial
Sealed vial with the freeze-dried cake. Check the label, lot and the certificate of analysis before opening.
Sterile diluent
The diluent named on the product documentation. Bacteriostatic water contains benzyl alcohol; sterile water does not.
Graduated syringes
Sized to the volume being measured. U-100 insulin syringes are graduated in units where 100 units = 1 mL.
Alcohol prep pads
For wiping vial stoppers before each puncture.
Nitrile gloves
Clean gloves for aseptic handling.
Sharps container
Puncture-resistant container for used needles.

Step-by-step reconstitution guide Storage and transport accessories

Storage

General research-context handling only: lyophilised short peptides are normally kept cold, dry and protected from light, and reconstituted material is far less stable than the dry powder. Amiravo gives no preparation, reconstitution or handling instructions; defer to the supplier's own documentation and to local institutional requirements for research materials.

Lyophilized (unmixed) vials: typically stored cold and dry, away from light, as the documentation directs. Many are stable longer frozen.
Reconstituted vials: typically refrigerated (about 2–8 °C), protected from light and not frozen.
Bacteriostatic water contains benzyl alcohol, which inhibits bacterial growth in multi-use vials; sterile water has no preservative.

Handling mistakes and troubleshooting

Added the wrong volume of diluent: The concentration changes, not the amount of compound in the vial. Recalculate with the volume actually added and relabel the vial.
Shook the vial and it foamed: Let it settle in the refrigerator. Foam is a sign of agitation; some peptides aggregate when shaken. Note it on the label.
The solution is cloudy or has particles: Do not use it. Cloudiness can indicate incomplete dissolution, contamination or degradation. Record the lot and contact the supplier with the certificate of analysis.
The powder cake looks melted or shrunken before reconstitution: This can indicate the vial was exposed to heat or moisture. Record the lot and condition on arrival.
Liquid sprayed back when the needle was removed: Pressure built up in the vial. Withdraw a small volume of air while adding diluent to keep pressure neutral.

Regulatory status

Status: Not approved by the FDA for any use; no approved application and no structured product label were identified ^6,7.
United States (FDA): No approved application for this substance was identified in Drugs@FDA, and no structured product label for it was identified in DailyMed ^6,7. It does not appear in any of the three categories of FDA's 503A bulk drug substances list as updated May 14, 2026 (Category 1, substances under evaluation; Category 2, substances raising significant safety risks; Category 3, substances nominated without adequate support) ^8.
Sport (WADA): The substance is not named anywhere in the WADA 2026 Prohibited List ^9. Absence of a name is not a clearance: several classes on the List are written as open categories using wording such as "including, but not limited to", and section S0 (Non-Approved Substances) is written to cover pharmacological substances that are not addressed elsewhere on the List and that have no current approval from a governmental health authority for human therapeutic use, a description that fits an unapproved research peptide ^9. Whether any particular substance falls inside S0 or inside an open class is a determination for anti-doping authorities, not something that can be read off the absence of a name.

Status varies by country and changes over time. How we describe regulatory status.

Pinealon compared with related compounds

Compound	Status	Evidence	Human amounts	Half-life
Pinealon	Not approved — research use only	C	None established	—
Sleep Research Blend	Not approved — research use only	—	None established	—
Vesugen	Not approved — research use only	C	None established	—
Testagen	Not approved — research use only	D	None established	—
Prostamax	Not approved — research use only	D	None established	—
Bronchogen	Not approved — research use only	C	None established	—

Frequently asked questions

What doses of Pinealon have been studied in humans?

None that establish an amount. Pinealon is not approved anywhere and we found no human studies that administered it. Amiravo does not publish community or vendor figures.

Is there an established Pinealon cycle length?

No cycle length or break has been established. Human studies administered Pinealon for the periods listed on this page; those are study durations, not a recommended course.

How much bacteriostatic water should be added to a Pinealon vial?

There is no compound-specific volume. The diluent volume only sets the concentration: concentration = mass in the vial ÷ volume added (for example, 10 mg in 2 mL gives 5 mg/mL). The calculator on this page shows the arithmetic for any combination.

How many units is a given amount on an insulin syringe?

On a U-100 syringe, 100 units = 1 mL. Units for an amount = amount ÷ (concentration per unit). The calculator converts any amount you enter at the concentration you choose; a unit is a volume mark, not an amount of compound.

Is Pinealon FDA-approved?

Not approved — research use only. Not approved by the FDA for any use; no approved application and no structured product label were identified ^6,7.

References

Pinealon increases cell viability by suppression of free radical levels and activating proliferative processes Rejuvenation Res. 2011
Pinealon protects the rat offspring from prenatal hyperhomocysteinemia Int J Clin Exp Med. 2012
EDR Peptide: Possible Mechanism of Gene Expression and Protein Synthesis Regulation Involved in the Pathogenesis of Alzheimer's Disease Molecules. 2020
Short peptides stimulate serotonin expression in cells of brain cortex Bull Exp Biol Med. 2014
Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA Biochemistry (Mosc). 2011
Drugs@FDA: FDA-Approved Drugs (search of approved applications) FDA Drugs@FDA / openFDA drug/drugsfda. 2026
DailyMed label search (structured product labels) NLM DailyMed. 2026
Bulk Drug Substances Nominated for Use in Compounding Under Section 503A of the FD&C Act, updated May 14, 2026 FDA. 2026
World Anti-Doping Code International Standard: Prohibited List 2026 WADA. 2026
Effect of short peptides on expression of signaling molecules in organotypic pineal cell culture Bull Exp Biol Med. 2011
[Analysis of some parameters of biological age and adaptation possibilities of workers of locomotive brigades] Adv Gerontol. 2012
[EFFECT OF SYNTHETIC PEPTIDES ON AGING OF PATIENTS WITH CHRONIC POLYMORBIDITY AND ORGANIC BRAIN SYNDROME OF THE CENTRAL NERVOUS SYSTEM IN REMISSION] Adv Gerontol. 2015
[Comparative analysis of different methods of geroprotective] Adv Gerontol. 2016
[The peptide correction of neurotic disorders among professional truck-drivers] Adv Gerontol. 2012

Last reviewed October 23, 2026 by the Amiravo editorial team. Editorial policy · Report an error