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Longevity · Dosing reference

N-Acetyl Epithalon Amidate dosage

What the human evidence does and does not show, how long studies ran, and the arithmetic for working with vials.

Not approved — research use onlyNone established
Full research pageCycle-length evidenceComparePrefer Amiravo on Google
Regulatory status
Not approved — research use only
Human amounts
None established in humans
Route studied
—
Frequency
Not established
Half-life
Not established
Evidence grade
Not yet assessed
Vial sizes on the market
Not recorded
Other forms seen
—

What N-Acetyl Epithalon Amidate is

A chemically modified analog of the tetrapeptide epithalon, studied in research on telomerase activity and cellular aging.

No mechanistic study of this modified analog was identified, so no mechanism is attributed to it 5. The rest of this section describes the parent peptide and is labelled as such. The parent peptide, epitalon Epitalon ( /library/epithalon ) is the tetrapeptide Ala-Glu-Asp-Gly 1,2. Reviews report that it has been studied for effects on telomere-maintenance pathways, circadian and neuroendocrine function, oxidative… How it works →

Key evidence

  • Jain et al., Int J Mol Sci · 2026 · Literature (epitalon and epithalamin, appraised separately)
    Concluded that epitalon is biologically active but clinically unproven, with human evidence limited to small physiological or biomarker studies and an older controlled retinitis pigmentosa study, inadequate pharmacokinetic and long-term safety data, and unresolved regulatory uncertainty after a 2026 FDA advisory review. The review concerns epitalon, not an acetylated or amidated analog. 1
  • Araj et al., Int J Mol Sci · 2025 · Literature (in vitro, in vivo and in silico studies of epitalon)
    Summarised about 25 years of work on the parent tetrapeptide, reported effects including on melatonin synthesis, interleukin-2 mRNA, thymocyte mitogenic activity and telomerase, and noted that physico-chemical and structural investigation of the peptide remains limited. 2
  • Khavinson et al., Neuro Endocrinol Lett · 2002 · Humans (patients with retinitis pigmentosa)
    Reported effects of the parent tetrapeptide on retinal condition; this is work from the St Petersburg Institute of Bioregulation and Gerontology, which developed the peptide, and concerns epitalon rather than any modified analog. 9
All studies on the research page

What the human evidence shows

No approved or established human dose

We searched PubMed and ClinicalTrials.gov and found no human studies that administered N-Acetyl Epithalon Amidate. The research described on its page is from cells or animals, and animal amounts do not translate to people.

How long did human studies run?

No human study durations are recorded for N-Acetyl Epithalon Amidate.

Cycle-length evidence for N-Acetyl Epithalon Amidate

N-Acetyl Epithalon Amidate draw calculator

Pick the vial strength and the water added, enter an amount, and see the concentration, the units to draw and how many draws the vial holds. Arithmetic only.

1 · Vial strength
2 · Water added
3 · Amount per draw
4 · Syringe
Your mix
10 mg + 2 mL water = 5 mg/mL
10 units holds 0.5 mg (500 mcg)
Draw toEnter an amount in step 3
0102030405060708090100
U-100 syringe · 100 units = 1 mL

Computed from your inputs. Not a dose or a recommendation.

Compare water volumes for this vialShow

More water = lower concentration = a larger, easier-to-measure draw. Tap a row to use it.

Watermg/mLDraw
0.5 mL20—
1 mL10—
1.5 mL6.667—
2 mL5—
2.5 mL4—
3 mL3.333—
5 mL2—

Supplies and handling

  • Lyophilized vial
    Sealed vial with the freeze-dried cake. Check the label, lot and the certificate of analysis before opening.
  • Sterile diluent
    The diluent named on the product documentation. Bacteriostatic water contains benzyl alcohol; sterile water does not.
  • Graduated syringes
    Sized to the volume being measured. U-100 insulin syringes are graduated in units where 100 units = 1 mL.
  • Alcohol prep pads
    For wiping vial stoppers before each puncture.
  • Nitrile gloves
    Clean gloves for aseptic handling.
  • Sharps container
    Puncture-resistant container for used needles.
Step-by-step reconstitution guideStorage and transport accessories

Storage

General research-context handling only. Lyophilized synthetic peptides are typically held cold and protected from light and moisture, and reconstituted material is less stable than lyophilized material; defer to the supplier's own stability and handling documentation for the specific lot. No preparation or administration instructions are given on this page.

  • Lyophilized (unmixed) vials: typically stored cold and dry, away from light, as the documentation directs. Many are stable longer frozen.
  • Reconstituted vials: typically refrigerated (about 2–8 °C), protected from light and not frozen.
  • Bacteriostatic water contains benzyl alcohol, which inhibits bacterial growth in multi-use vials; sterile water has no preservative.

Handling mistakes and troubleshooting

Added the wrong volume of diluent
The concentration changes, not the amount of compound in the vial. Recalculate with the volume actually added and relabel the vial.
Shook the vial and it foamed
Let it settle in the refrigerator. Foam is a sign of agitation; some peptides aggregate when shaken. Note it on the label.
The solution is cloudy or has particles
Do not use it. Cloudiness can indicate incomplete dissolution, contamination or degradation. Record the lot and contact the supplier with the certificate of analysis.
The powder cake looks melted or shrunken before reconstitution
This can indicate the vial was exposed to heat or moisture. Record the lot and condition on arrival.
Liquid sprayed back when the needle was removed
Pressure built up in the vial. Withdraw a small volume of air while adding diluent to keep pressure neutral.

Regulatory status

Status
Not approved by the FDA for any use, and a DailyMed name search for epitalon returned no drug label 6,7. Neither epitalon nor any acetylated or amidated analog appears in category 1, 2 or 3 of FDA's May 14, 2026 503A bulk drug substances list 8. A 2026 narrative review of epitalon notes unresolved regulatory uncertainty following an FDA advisory review in 2026 1.
United States (FDA)
No approved FDA application exists for epitalon or for any acetylated or amidated analog, and a DailyMed name search for epitalon returned no drug label 6,7. Neither appears in category 1, 2 or 3 of FDA's May 14, 2026 503A bulk drug substances list 8. A 2026 narrative review refers to regulatory uncertainty following an FDA advisory review in 2026 as unresolved 1.
Sport (WADA)
Neither epitalon, nor epithalon, nor any acetylated or amidated analog is named in the WADA 2026 Prohibited List 13. That absence is hedged negative evidence and not a clearance: section S0, Non-Approved Substances, prohibits at all times any pharmacological substance not addressed by a later section and with no current approval by any governmental regulatory health authority for human therapeutic use, and introduces its examples with "including but not limited to" 13. A modified analog with no approval anywhere falls squarely within the situation that section describes, so an athlete should not infer permission from the absence of a name 13.

Status varies by country and changes over time. How we describe regulatory status.

N-Acetyl Epithalon Amidate compared with related compounds

CompoundStatusEvidenceHuman amountsHalf-life
N-Acetyl Epithalon AmidateNot approved — research use only—None established—
EpithalonNot approved — research use onlyCNone established—
VesugenNot approved — research use onlyCNone established—
CartalaxNot approved — research use onlyDNone established—
ChonlutenNot approved — research use onlyDNone established—
Methylene BlueFDA-approvedAFDA labelApproximately 24 hours

Frequently asked questions

What doses of N-Acetyl Epithalon Amidate have been studied in humans?
None that establish an amount. N-Acetyl Epithalon Amidate is not approved anywhere and we found no human studies that administered it. Amiravo does not publish community or vendor figures.
Is there an established N-Acetyl Epithalon Amidate cycle length?
No. We found no human studies that report treatment duration for N-Acetyl Epithalon Amidate.
How much bacteriostatic water should be added to a N-Acetyl Epithalon Amidate vial?
There is no compound-specific volume. The diluent volume only sets the concentration: concentration = mass in the vial ÷ volume added (for example, 10 mg in 2 mL gives 5 mg/mL). The calculator on this page shows the arithmetic for any combination.
How many units is a given amount on an insulin syringe?
On a U-100 syringe, 100 units = 1 mL. Units for an amount = amount ÷ (concentration per unit). The calculator converts any amount you enter at the concentration you choose; a unit is a volume mark, not an amount of compound.
Is N-Acetyl Epithalon Amidate FDA-approved?
Not approved — research use only. Not approved by the FDA for any use, and a DailyMed name search for epitalon returned no drug label 6,7. Neither epitalon nor any acetylated or amidated analog appears in category 1, 2 or 3 of FDA's May 14, 2026 503A bulk drug substances list 8. A 2026 narrative review of epitalon notes unresolved regulatory uncertainty following an FDA advisory review in 2026 1.

References

  1. Epitalon and Epithalamin: A Narrative Review of Mechanistic, Preclinical, and Clinical Evidence Int J Mol Sci. 2026
  2. Overview of Epitalon-Highly Bioactive Pineal Tetrapeptide with Promising Properties Int J Mol Sci. 2025
  3. PubChem compound name search for "N-Acetyl Epithalon Amidate" and variants returned no CID (searched 22 October 2026) PubChem. 2026
  4. Epitalon (CID 219042): molecular formula C14H22N4O9, molecular weight 390.35; a name search for "Epithalamin" also resolves to this record PubChem. 2026
  5. PubMed searches returning zero records: "N-acetyl epithalon amidate", "epithalon amidate", "acetylated epitalon", AEDG AND amide (searched 22 October 2026) PubMed. 2026
  6. Therapeutic peptides in gerontology: mechanisms and applications for healthy aging Front Aging. 2026
  7. DailyMed drug name search returned no labels for epitalon (searched 22 October 2026) DailyMed. 2026
  8. Bulk Drug Substances Nominated for Use in Compounding Under Section 503A of the Federal Food, Drug, and Cosmetic Act (updated May 14, 2026) FDA. 2026
  9. Pineal-regulating tetrapeptide epitalon improves eye retina condition in retinitis pigmentosa Neuro Endocrinol Lett. 2002
  10. Systemic and Topical Geroprotective Rejuvenation Assessed by START/REST Aging Clocks (NCT07888595), early phase 1, enrolling by invitation ClinicalTrials.gov. 2026
  11. LCP2 mediates SUV39H1-driven cellular senescence-related chemoresistance in natural killer/T-cell lymphoma Cell Death Dis. 2026
  12. Therapeutic Peptides in Orthopaedics: Applications, Challenges, and Future Directions J Am Acad Orthop Surg Glob Res Rev. 2026
  13. World Anti-Doping Code International Standard: Prohibited List 2026 WADA. 2026

Last reviewed October 23, 2026 by the Amiravo editorial team. Editorial policy · Report an error