Longevity · Dosing reference

Epithalon dosage

What the human evidence does and does not show, how long studies ran, and the arithmetic for working with vials.

Not approved — research use onlyCPreclinical researchNone established

Full research page Cycle-length evidence Compare Prefer Amiravo on Google

Regulatory status: Not approved — research use only
Human amounts: None established in humans
Route studied: —
Frequency: Not established
Half-life: Not established
Evidence grade: Grade C · Preclinical research
Vial sizes on the market: 10 mg · 40 mg · 50 mg
Other forms seen: —

What Epithalon is

Epithalon is a synthetic tetrapeptide modeled on a pineal gland extract and investigated in cell and animal research on telomerase, antioxidant activity and aging. Most of the literature originates from a small number of research groups. It is not an approved medicine in the United States.

Epithalon (also written Epitalon) is the tetrapeptide Ala-Glu-Asp-Gly (AEDG), synthesized from the amino acid composition of Epithalamin, a bovine pineal gland extract ^2. Proposed mechanisms Telomerase: In telomerase-negative human fetal fibroblast cultures, it was reported to induce expression of the catalytic subunit of telomerase, telomerase enzymatic activity and telomere elongation ^1. Antioxidant and… How it works →

Key evidence

Khavinson et al., Bull Exp Biol Med · 2003 · Cells (human fetal fibroblast culture)
Addition of Epithalon to telomerase-negative fibroblasts was reported to induce telomerase catalytic subunit expression, enzymatic activity and telomere elongation. ¹
Araj et al., Int J Mol Sci · 2025 · Review of in vitro, in vivo and in silico studies
The review summarizes reported antioxidant, neuroprotective, antimutagenic and neuroendocrine effects and states that the mechanisms remain uncertain and structural data are limited. ²
Khavinson, Neuro Endocrinol Lett · 2002 · Animals and humans (as reported by the originating group)
Reports lifespan extension in mice and fruit flies and restored melatonin and cortisol rhythms in old rhesus monkeys; also describes clinical studies in pigmentary retinal degeneration. ³

All studies on the research page

What the human evidence shows

No approved or established human dose

We searched PubMed and ClinicalTrials.gov and found no human studies that administered Epithalon. The research described on its page is from cells or animals, and animal amounts do not translate to people.

How long did human studies run?

No human study durations are recorded for Epithalon.

Cycle-length evidence for Epithalon

Epithalon draw calculator

Pick the vial strength and the water added, enter an amount, and see the concentration, the units to draw and how many draws the vial holds. Arithmetic only.

1 · Vial strength

sizes on the market

2 · Water added

3 · Amount per draw

4 · Syringe

Your mix

10 mg + 2 mL water = 5 mg/mL

10 units holds 0.5 mg (500 mcg)

Draw toEnter an amount in step 3

U-100 syringe · 100 units = 1 mL

Computed from your inputs. Not a dose or a recommendation.

Compare water volumes for this vialShow

More water = lower concentration = a larger, easier-to-measure draw. Tap a row to use it.

Water	mg/mL	Draw
0.5 mL	20	—
1 mL	10	—
1.5 mL	6.667	—
2 mL	5	—
2.5 mL	4	—
3 mL	3.333	—
5 mL	2	—

Supplies and handling

Lyophilized vial
Sealed vial with the freeze-dried cake. Check the label, lot and the certificate of analysis before opening.
Sterile diluent
The diluent named on the product documentation. Bacteriostatic water contains benzyl alcohol; sterile water does not.
Graduated syringes
Sized to the volume being measured. U-100 insulin syringes are graduated in units where 100 units = 1 mL.
Alcohol prep pads
For wiping vial stoppers before each puncture.
Nitrile gloves
Clean gloves for aseptic handling.
Sharps container
Puncture-resistant container for used needles.

Step-by-step reconstitution guide Storage and transport accessories

Storage

Lyophilized peptides are generally handled according to the supplier's documentation, typically protected from light and moisture; follow the stability data supplied with the specific lot.

Lyophilized (unmixed) vials: typically stored cold and dry, away from light, as the documentation directs. Many are stable longer frozen.
Reconstituted vials: typically refrigerated (about 2–8 °C), protected from light and not frozen.
Bacteriostatic water contains benzyl alcohol, which inhibits bacterial growth in multi-use vials; sterile water has no preservative.

Handling mistakes and troubleshooting

Added the wrong volume of diluent: The concentration changes, not the amount of compound in the vial. Recalculate with the volume actually added and relabel the vial.
Shook the vial and it foamed: Let it settle in the refrigerator. Foam is a sign of agitation; some peptides aggregate when shaken. Note it on the label.
The solution is cloudy or has particles: Do not use it. Cloudiness can indicate incomplete dissolution, contamination or degradation. Record the lot and contact the supplier with the certificate of analysis.
The powder cake looks melted or shrunken before reconstitution: This can indicate the vial was exposed to heat or moisture. Record the lot and condition on arrival.
Liquid sprayed back when the needle was removed: Pressure built up in the vial. Withdraw a small volume of air while adding diluent to keep pressure neutral.

Regulatory status

Status: Not approved by the FDA for any use; no FDA label was identified for this compound. It has been studied in cell and animal research, with limited human reports from its originating institute ^2,3.
United States (FDA): No FDA-approved label for Epithalon was identified; it is not approved for any indication ^2.
Sport (WADA): Epithalon is not named in the 2026 WADA Prohibited List text reviewed; Section S0 covers pharmacological substances with no approval by any governmental regulatory authority for human therapeutic use, so athletes should confirm status with their anti-doping organization ^5.

Status varies by country and changes over time. How we describe regulatory status.

Epithalon compared with related compounds

Compound	Status	Evidence	Human amounts	Half-life
Epithalon	Not approved — research use only	C	None established	—
Cartalax	Not approved — research use only	D	None established	—
N-Acetyl Epithalon Amidate	Not approved — research use only	—	None established	—
Chonluten	Not approved — research use only	D	None established	—
Methylene Blue	FDA-approved	A	FDA label	Approximately 24 hours
L-Glutathione	Not approved — research use only	B	None established	—

Frequently asked questions

What doses of Epithalon have been studied in humans?

None that establish an amount. Epithalon is not approved anywhere and we found no human studies that administered it. Amiravo does not publish community or vendor figures.

Is there an established Epithalon cycle length?

No. We found no human studies that report treatment duration for Epithalon.

How much bacteriostatic water should be added to a Epithalon vial?

There is no compound-specific volume. The diluent volume only sets the concentration: concentration = mass in the vial ÷ volume added (for example, 10 mg in 2 mL gives 5 mg/mL). The calculator on this page shows the arithmetic for any combination.

How many units is a given amount on an insulin syringe?

On a U-100 syringe, 100 units = 1 mL. Units for an amount = amount ÷ (concentration per unit). The calculator converts any amount you enter at the concentration you choose; a unit is a volume mark, not an amount of compound.

Is Epithalon FDA-approved?

Not approved — research use only. Not approved by the FDA for any use; no FDA label was identified for this compound. It has been studied in cell and animal research, with limited human reports from its originating institute ^2,3.

References

Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells Bull Exp Biol Med. 2003
Overview of Epitalon-Highly Bioactive Pineal Tetrapeptide with Promising Properties Int J Mol Sci. 2025
Peptides and Ageing Neuro Endocrinol Lett. 2002
Epitalon protects against post-ovulatory aging-related damage of mouse oocytes in vitro Aging (Albany NY). 2022
WADA 2026 Prohibited List World Anti-Doping Agency. 2026

Last reviewed October 22, 2026 by the Amiravo editorial team. Editorial policy · Report an error